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affinity bf9103 cleaved caspase  (Proteintech)


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    Structured Review

    Proteintech affinity bf9103 cleaved caspase
    Affinity Bf9103 Cleaved Caspase, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 4864 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase+3+affinity/Caspase+3%2Fp17%2Fp19+Antibody/pmc12559320__41467_2025_64518_MOESM1_ESM-85-21-32
    Average 97 stars, based on 4864 article reviews
    affinity bf9103 cleaved caspase - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Expressing:

    Article Title: Breaking the Iron Homeostasis: A "Trojan Horse" Self-Assembled Nanodrug Sensitizes Homologous Recombination Proficient Ovarian Cancer Cells to PARP Inhibition.
    Article Snippet: .. SKOV3 and OVCAR3 cells were analyzed for the expression of RRM2 (Proteintech), γ-H2AX (Abcam), HMOX1 (Proteintech), PI3K (Cell Signaling Technology), p-PI3K (Cell Signaling Technology), AKT (Abcam), p-ATK (Cell Signaling Technology), BAX (Proteintech), cleaved-caspase 3 (Affinity), and β-actin (Proteintech) proteins, as previously described.36 The cells were collected and lysed after treatment (control, olaparib, or olaparib-Ga). .. Subsequently, the protein was transferred to PVDF membranes (Millipore), and Western blot analysis was performed with antibodies according to the manufacturer’s recommendations.

    Article Title: Breaking the Iron Homeostasis: A “Trojan Horse” Self-Assembled Nanodrug Sensitizes Homologous Recombination Proficient Ovarian Cancer Cells to PARP Inhibition
    Article Snippet: .. SKOV3 and OVCAR3 cells were analyzed for the expression of RRM2 (Proteintech), γ-H2AX (Abcam), HMOX1 (Proteintech), PI3K (Cell Signaling Technology), p-PI3K (Cell Signaling Technology), AKT (Abcam), p-ATK (Cell Signaling Technology), BAX (Proteintech), cleaved-caspase 3 (Affinity), and β-actin (Proteintech) proteins, as previously described. ..

    Control:

    Article Title: Breaking the Iron Homeostasis: A "Trojan Horse" Self-Assembled Nanodrug Sensitizes Homologous Recombination Proficient Ovarian Cancer Cells to PARP Inhibition.
    Article Snippet: .. SKOV3 and OVCAR3 cells were analyzed for the expression of RRM2 (Proteintech), γ-H2AX (Abcam), HMOX1 (Proteintech), PI3K (Cell Signaling Technology), p-PI3K (Cell Signaling Technology), AKT (Abcam), p-ATK (Cell Signaling Technology), BAX (Proteintech), cleaved-caspase 3 (Affinity), and β-actin (Proteintech) proteins, as previously described.36 The cells were collected and lysed after treatment (control, olaparib, or olaparib-Ga). .. Subsequently, the protein was transferred to PVDF membranes (Millipore), and Western blot analysis was performed with antibodies according to the manufacturer’s recommendations.

    BIA-KA:

    Article Title: CREB1 protects against the renal injury in a rat model of kidney stone disease and calcium oxalate monohydrate crystals-induced injury in NRK-52E cells.
    Article Snippet: Kidney stone disease (KSD) is a common urinary disease with increasing prevalence worldwide.. In this study, we investigated the effect of cyclic AMP responsive element binding protein (CREB) 1 in a KSD model of rat and calcium oxalate monohydrate (COM) crystals-treated NRK-52E cells.. Rats were pretreated with lentivirus (LV)CREB1 vector or LV-control vector and administrated with ethylene glycol + ammonium chloride to induce KSD.

    SDS Page:

    Article Title: CREB1 protects against the renal injury in a rat model of kidney stone disease and calcium oxalate monohydrate crystals-induced injury in NRK-52E cells.
    Article Snippet: Kidney stone disease (KSD) is a common urinary disease with increasing prevalence worldwide.. In this study, we investigated the effect of cyclic AMP responsive element binding protein (CREB) 1 in a KSD model of rat and calcium oxalate monohydrate (COM) crystals-treated NRK-52E cells.. Rats were pretreated with lentivirus (LV)CREB1 vector or LV-control vector and administrated with ethylene glycol + ammonium chloride to induce KSD.

    Membrane:

    Article Title: CREB1 protects against the renal injury in a rat model of kidney stone disease and calcium oxalate monohydrate crystals-induced injury in NRK-52E cells.
    Article Snippet: Kidney stone disease (KSD) is a common urinary disease with increasing prevalence worldwide.. In this study, we investigated the effect of cyclic AMP responsive element binding protein (CREB) 1 in a KSD model of rat and calcium oxalate monohydrate (COM) crystals-treated NRK-52E cells.. Rats were pretreated with lentivirus (LV)CREB1 vector or LV-control vector and administrated with ethylene glycol + ammonium chloride to induce KSD.

    Incubation:

    Article Title: CREB1 protects against the renal injury in a rat model of kidney stone disease and calcium oxalate monohydrate crystals-induced injury in NRK-52E cells.
    Article Snippet: Kidney stone disease (KSD) is a common urinary disease with increasing prevalence worldwide.. In this study, we investigated the effect of cyclic AMP responsive element binding protein (CREB) 1 in a KSD model of rat and calcium oxalate monohydrate (COM) crystals-treated NRK-52E cells.. Rats were pretreated with lentivirus (LV)CREB1 vector or LV-control vector and administrated with ethylene glycol + ammonium chloride to induce KSD.

    Article Title: Isobavachalcone alleviates ischemic stroke by suppressing HDAC1 expression and improving M2 polarization.
    Article Snippet: The proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Abcam). .. Subsequently, the membranes were blocked with blocking solution for 60 min (containing 1–5 % BSA, Beyotime, P0023) and incubated with primary antibodies against cleaved caspase 3 (Affinity), BAX (Affinity), Bcl-2 (Affinity), iNOS (Affinity), CD86 (Proteintech), Arg-1 (Affinity), CD206 (Affinity), HDAC1 (Affinity). .. Next, the membranes were washed with western washing liquid (Beyotime) and incubated with goat-antirabbit IgG or goat-anti-mouse IgG (Beyotime).

    Blocking Assay:

    Article Title: Isobavachalcone alleviates ischemic stroke by suppressing HDAC1 expression and improving M2 polarization.
    Article Snippet: The proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Abcam). .. Subsequently, the membranes were blocked with blocking solution for 60 min (containing 1–5 % BSA, Beyotime, P0023) and incubated with primary antibodies against cleaved caspase 3 (Affinity), BAX (Affinity), Bcl-2 (Affinity), iNOS (Affinity), CD86 (Proteintech), Arg-1 (Affinity), CD206 (Affinity), HDAC1 (Affinity). .. Next, the membranes were washed with western washing liquid (Beyotime) and incubated with goat-antirabbit IgG or goat-anti-mouse IgG (Beyotime).



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    Affinity Biosciences polyclonal rabbit antibody for pro-apoptotic cleaved-caspase 3 affinity biosciences cat# af7022
    (A) The effects of drugs on tumour cell apoptosis. Green spots represent <t>apoptotic</t> bodies, and blue spots represent cell nuclei (400×). (B) The effects of drugs on the expression of cleaved caspase-3 in tumour tissue (400×). (C) Cleaved caspase 3 and corresponding frequencies of TUNEL positive cells expressed as AI. Different marks within treatments indicate significant differences at ∗ p < 0.05 and ∗∗ p < 0.01 compared to the TC group (n = 3). (D) Weight change trends in 10 acutely toxic experimental mice.
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    Image Search Results


    (A) The effects of drugs on tumour cell apoptosis. Green spots represent apoptotic bodies, and blue spots represent cell nuclei (400×). (B) The effects of drugs on the expression of cleaved caspase-3 in tumour tissue (400×). (C) Cleaved caspase 3 and corresponding frequencies of TUNEL positive cells expressed as AI. Different marks within treatments indicate significant differences at ∗ p < 0.05 and ∗∗ p < 0.01 compared to the TC group (n = 3). (D) Weight change trends in 10 acutely toxic experimental mice.

    Journal: Heliyon

    Article Title: An in vivo and in vitro assessment of the anti-breast cancer activity of crude extract and fractions from Prunella vulgaris L.

    doi: 10.1016/j.heliyon.2022.e11183

    Figure Lengend Snippet: (A) The effects of drugs on tumour cell apoptosis. Green spots represent apoptotic bodies, and blue spots represent cell nuclei (400×). (B) The effects of drugs on the expression of cleaved caspase-3 in tumour tissue (400×). (C) Cleaved caspase 3 and corresponding frequencies of TUNEL positive cells expressed as AI. Different marks within treatments indicate significant differences at ∗ p < 0.05 and ∗∗ p < 0.01 compared to the TC group (n = 3). (D) Weight change trends in 10 acutely toxic experimental mice.

    Article Snippet: Tumour tissue sections were stained with polyclonal rabbit antibody for pro-apoptotic cleaved-caspase 3 (Affinity Biosciences Cat# AF7022, RRID: AB_2835326 ), according to the manufacturer's instructions.

    Techniques: Expressing, TUNEL Assay